【摘要】 目的研究同种异体骨髓间质干细胞(bone marrow mesenchymal stem cells MSCs)移植至梗死心肌后对兔的一般情况、心功能、梗死面积和血管新生的影响。方法开胸结扎冠状动脉左前降支建立急性心肌梗死动物模型,将5-溴脱氧尿核苷(bromodeoxyuridine,BrdU)标记的MSCs注入心肌梗死区。测定各组手术前后体重和心功能并取出心脏作相关检查。结果与急性心肌梗死组比较,MSCs组术后一般情况好,6周时体重明显增加(P<0.05),术后3周、6周左心室射血分数(left ventricular ejection fraction,LVEF)均明显增加(P<0.05),梗死面积明显缩小(P<0.05),毛细血管数目显著增多(P<0.01)。MSCs组心肌梗死区可见BrdU阳性细胞,电镜下可见不成熟的心肌样细胞。结论同种异体MSCs移植治疗急性心肌梗死除提高心功能外,尚可改善兔子的一般情况,增加体重。更多还原

【Abstract】 Objectives To study effect of exogenous mesenchymal stem cells(MSCs) transplantation for acute myocardial infarction(AMI) on general state of health, heart function,sizes of infarction, neovascularization in rabbits. Methods AMI was created by ligating left anterior descending (LAD) coronary artery. MSCs isolated from bone marrow and labeled with bromodeoxyuridine (BrdU) were injected into the infarcted myocardium. Weight and heart function were measured at pre-operation, 3nd, 6th week after operation. The hearts were harvested for detection. Results MSCs group took a favorable turn in general state of health and had a significantly incremental weight at 6 week after operation compared with AMI group (P < 0.05). A more significant improvement of LVEF was observed in the MSCs group than in AMI the group at 3 weeks and 6 weeks after transplantation (P < 0.05). Infarct size was significant smaller in the MSCs group than in the AMI group (P < 0.05). The capillary density of MSCs group was significantly increased than that of AMI group (P < 0.01). BrdU-positive cells and some immature cell were seen in the infarct myocardium in MSCs group. Conclusions Exogenous MSCs transplantation for myocardial infarction can improve general state of health and heart function and gain weight in rabbits.更多还原