【摘要】 利用鲁氏毛霉(Mucor rouxii)3.2545发酵产生壳聚糖,然后用氯磺酸/甲酰胺方法对壳聚糖进行硫酸酯化,当底物壳聚糖用量为1g时,其最佳反应条件为:10mL甲酰胺中氯磺酸添加量为3.0mL;反应温度为70℃;反应时间为3.0h。其中反应温度对反应的影响最大。在最适反应条件下,壳聚糖硫酸酯的硫含量可以达到16.04%。通过红外光谱分析,发现壳聚糖已经成功地被硫酸酯化,而且结构与肝素相似。此外,所得产物还具有抗凝血和抗血栓的性质,其抗血栓的性质优于肝素。更多还原

【Abstract】 The chitosan could be obtained by fermentation with Mucor rouxianus,and then product was sulfated by chlorosulfonic acid/formamide method.The optimal reaction condition was at 70 ℃ for 3.0h with 3.0mL chlorosulfonic acid as sulfated reagent and the most sulfur content was 16.04%.The effect of temperature on this reaction was more than that of others,so the former must be strictly controlled.The analysis of infra-red spectrum proved that chitosan were sulfated successfully and its construction was similar to heparin.Furthermore,sulfated chitosan also showed anticoagulant activity and anti-thrombogenicity which was better than that of heparin.In conclusion,sulfated derivatives of chitosan from Mucor rouxianus is likely to instead of heparin.更多还原