【摘要】 目的探讨血清白细胞介素12(IL-12)、白细胞介素12p70(IL-12p70)、白细胞介素10(IL-10)和白细胞介素18(IL-18)水平在Ⅰ期尘肺发生发展中的作用。方法采用双抗体夹心酶联免疫吸附测定(ELISA)法检测79例Ⅰ期尘肺患者(其中包括44例煤工尘肺患者和35例矽肺患者)、42例具有相同接尘史但未患尘肺的井下煤矿工人(接尘对照)及41例井上健康查体人员(正常对照)的血清IL-12、IL-12p70、IL-10和IL-18的含量。结果矽肺组IL-12和IL-10水平明显高于两对照组,组间差异有统计学意义(均P<0.05);煤工尘肺组IL-12和IL-10水平虽高于两对照组,但差异均无统计学意义(P>0.05);煤工尘肺组和矽肺组IL-12p70水平较两对照组均明显增高,差异具有统计学意义(P<0.01);而IL-18水平在各组间无明显改变(P>0.05)。煤工尘肺Ⅰ期、矽肺Ⅰ期及其对应的Ⅰ期合并高血压、冠心病和Ⅰ期并发慢性阻塞性肺疾病中4项指标水平比较,其差异无统计学意义(均P>0.05);煤工尘肺Ⅰ期和矽肺Ⅰ期患者不同接尘年限间比较,4项指标水平均未发现明显改变(P>0.05)。Ⅰ期尘肺患者血清IL-12、IL-12p70及IL-10三者之间呈正相关,而与IL-18均无相关性。结论Ⅰ期尘肺患者体内细胞因子网络紊乱可能与尘肺发病机制有关。更多还原

【Abstract】 Objective To explore the change of serum interleukin-12(IL-12),interleukin- 12p70(IL-12p70),interleukin-10(IL-10) and interleukin-18(IL-18) and its possible role in the occurrence and development of pneumoconiosis stageⅠ. Methods Serum levels of IL-12,IL-12p70,IL-10 and IL-18 were determined in 79 patients with pneumoconiosis stageⅠ,including 44 cases of coal workers’ pneumoconiosis(CWP group) and 35 cases of silicosis,besides,42 healthy coal workers under well and 41 healthy controls above well by a sandwich enzyme-linked immunosorbent assay(ELISA). Results Compared with two control groups,serum levels of IL-12 and IL-10 in silicosis group were significantly higher,respectively(all P<0.05); serum levels of IL-12 and IL-10 in CWP group were a little higher,but no significant difference(P>0.05); serum levels of IL-12p70 in silicosis and CWP group were significantly higher(P<0.01); however serum level of IL-18 had no significant difference among different groups(P>0.05).No significant difference was observed in serum IL-12,IL-12p70,IL-10 and IL-18 among CWP stage Ⅰ,silicosis stage Ⅰ and their complications(all P>0.05); There were no relationships between the four parameters and the duration of exposure to dust in CWP stage Ⅰand silicosis stage Ⅰ(P>0.05,respectively); There was positive correlation among serum levels of IL-12,IL-12p70 and IL-10 in pneumoconiosis stage Ⅰ,however there was no correlation between IL-18 and other three parameters. Conclusion The pathogenesis of pneumoconiosis stage Ⅰ was possibly associated with the apparent disturbance of the regulation networks of cytokines systems.更多还原