【摘要】 以Poly(I)-Poly(C)诱导鸡成纤维细胞Mx基因的表达,提取总RNA,RT-PCR扩增出全长Mx cDNA,扩增产物克隆入pMD19-T Simple载体中进行序列测定。结果表明:与GenBank公布的鸡Mx cDNA序列相比,其同源性达99.9%,为进一步研究鸡Mx基因的抗病毒活性和作用机理奠定了基础。更多还原

【Abstract】 A full-length cDNA of chicken Mx gene was obtained using reverse transcription polymerase chain reaction(RT-PCR) amplification of total RNA extracted from chicken embryo fibroblast(CEF) which was induced with Poly(I)-Poly(C).The RT-PCR product was subcloned into pMD19-T simple vector and the correctness of the sequences was confirmed by sequence analysis.Result of sequence analysis showed that its homology with Mx cDNA from GenBank was up to 99.9%.These data indicated that the fulllength Mx cDNA was cloned correctly,which will play a great role in research of its antiviral function and mechanism.更多还原