【摘要】 为了获得安全性较好的生长抑素（SS）基因疫苗,采用PCR扩增pcS/SS中的SS基因,将其融合到pUSS/S质粒S基因后,构建pUS/2SS质粒;然后将S/2SS融合基因亚克隆到pEGFP-N₁中,得到S/2SS融合表达质粒pES/2SS。酶切和测序鉴定表明:重组质粒pES/2SS构建成功。将pES/2SS转染HeLa细胞,用ELISA检测表达的融合蛋白可与SS抗体特异性结合;将pES/2SS免疫10只大鼠（每只100μg）后,5只大鼠产生抗SS抗体,而对照组未检出SS抗体。结果证明,所构建的质粒pES/2SS可在真核细胞中表达生长抑素,用于免疫可产生抗SS特异性抗体。更多还原

【Abstract】 To improve the safety of the somatostatin（SS）gene vaccine,a new expression plasmid pES/2SS as follows were constructed.SS gene in pcS/SS was amplified by PCR and was then fused to close behind the S gene of plasmid pUSS/S to obtain the pUS/2SS plasmid.Subsequently the fused gene of S/2SS was subcloned into pEGFP-N₁ to get a S/ 2SS recombinant expression plasmid pES/2SS.These plasmids were identified by restriction endonuclease digestion and se- quencing.The result showed that the pES/2SS was constructed successfully.The pES/2SS was then used to transfect HeLa cells through liposome embedding.The fused protein reacted with the antibodies against somatostatin by ELISA.Ten rats were immunized with 100μg of pES/2SS.Five of them produced antibodies against SS in vivo.In conclusion,the plas- mid pES/2SS can express SS in eukaryotes and be used to produce SS antibodies after immunization.更多还原