【摘要】 目的观察大鼠发情周期各期下丘脑中食欲素及其受体(OX1R、OX2R)表达的变化,探讨食欲素对发情周期的可能调节作用。方法通过竞争性逆转录多聚酶链式反应(competitive RT-PCR)方法检测大鼠下丘脑中食欲素前体(Prepro-Orexin)、食欲素-A、OXIR和OX2R在发情周期各期的表达。结果发情前期的OX1R mRNA表达水平明显高于发情后期,而prepro-OX和OX2R mRNA的表达各期间没有明显的不同。结论食欲素可能通过结合OX1R调节促性腺激素释放激素和/或黄体生成素的分泌而参与排卵的发生。更多还原

【Abstract】 Objective To investigate a possible role of orexin in the regulation of estrous cycle by examining the expression of orexin and orexin receptors(OX1R,OX2R) in the rat hypothalamus during the estrous cycle. Methods The levels of prepro-orexin(prepro-OX),orexin-A,OX1R,and OX2R in the rat hypothalamus during the estrous cycle were evaluated by competitive reverse transcription-polymerase china reaction(RT-PCR) assay. Results Only expression OX1R mRNA during late proestrus was significantly higher than that at metestrus.No significant changes of prepro-OX and OX2R mRNA expression were observed during the various estrous cycle phases.Conclusion Orexin might regulate the secretion of GnRH and/or LH by binding OX1R,contributing to the occurrence of ovulation.更多还原