【摘要】 目的观察蛋白激酶Cγ亚型(PKCγ)在成年大鼠延髓和脊髓白质内的分布。方法免疫组织化学ABC法显示PKCγ免疫阳性物质在延髓和脊髓颈、胸、腰、骶段白质内的表达和分布。结果PKCγ免疫阳性物质分布于锥体、锥体交叉及脊髓颈、胸、腰、骶段后索腹侧中;延髓和脊髓背角神经元中也有PKCγ免疫阳性物质存在。结论PKCγ免疫阳性反应产物广泛分布于成年大鼠延髓和脊髓白质内的皮质脊髓束中。PKCγ免疫阳性反应产物在延髓锥体和脊髓后索内的定位与大鼠皮质脊髓束的位置和走行一致,提示其在运动神经传导通路中可能起着重要作用。更多还原

【Abstract】 Objective To investigate the distribution of protein kinase C gamma(PKCγ) in medulla oblongata and white matter of the spinal cord in adult rats. Methods Immunohistochemical staining technique(ABC method) was used to demonstrate the localization of PKCγ immunoreactivities. Results PKCγ immunoreactivities were located in the pyramid,decussation of pyramid and the ventral part of posterior funiculus in the cervical,thoracic,lumbar and sacral segments of the spinal cord.In addition,PKCγ immunoreactivities were also expressed in the neurons at dorsal horn of medullary and different segments of the spinal cord.Conclusion The distribution of PKCγ in medulla oblongata and white matter of the spinal cord in adult rats are correspondent with the position and course of the rat corticospinal tract.The widespread expression of PKCγ in corticospinal tract in adult rats indicates that it might play an important role in the motorneuron pathway.更多还原