【摘要】 目的探讨小鼠杏仁核中的亨廷顿蛋白相关蛋白HAP1(huntingtin associated protein 1,HAP1)的分布及是否与饥饿有关。方法采用半定量免疫印迹法(Western blotting)检测禁食过程小鼠杏仁核HAP1表达的变化。应用免疫组织化学染色法观察禁食对杏仁核HAP1表达的影响。结果连续禁食1日、2日、3日、4日的小鼠杏仁核的HAP1含量与正常进食的小鼠相比,呈逐渐增多的趋势。其HAP1阳性细胞的表达随禁食时间的延长显著增高。结论HAP1与饥饿有关,并有可能参与了食欲的调节。更多还原

【Abstract】 Objective: To probe for if huntingtin associated protein 1(HAP1) is involved in hungry.Methods: Western blotting was used to observe the effect of food-deprivation on expressing of HAP1 in the amygdala of the mouse.Method of immunohistochemistry was used to determine the change of HAP1 in the amygdala.Results: It was detected by western blotting that the HAP1 expression in the amygdala increases when food-deprivation continued.By immunohistochemistry,HAP1 immunoreactivity was shown to enhanced in the amygdala.Conclusion: HAP1 might be involved in hungry and participate in appetite regulation.更多还原