【摘要】 目的研究中药金樱子对糖尿病大鼠肾脏的保护作用并探讨其保护机制。方法用链脲佐菌素(streptozotocin,STZ)腹腔注射诱导法建立SD大鼠糖尿病模型,随机分为糖尿病模型组和金樱子干预组,同时另设正常对照组和金樱子对照组。观察大鼠一般状态,测定血糖、体重(BW)及肾重(KW)、肾脏指数(KW/BW),肾组织光镜检查了解组织形态,肾组织免疫组化检查观察金樱子对糖尿病大鼠肾脏组织转化生长因子1β(TGF-1β),Ⅳ型胶原表达影响。结果糖尿病模型组血糖及肾脏指数明显升高,肾脏病理改变明显,TGF-β1、Ⅳ型胶原表达明显增加。治疗24周后,金樱子干预组血糖降低,肾脏肥大明显改善,TGF-1β,Ⅳ型胶原表达明显下降(P<0.05),肾脏病理改变较糖尿病模型组有较大改善,但金樱子对照组肾组织无明显病理改变。结论金樱子对糖尿病大鼠肾脏有保护作用,其机制与抑制糖尿病大鼠肾脏TGF-β1,Ⅳ型胶原蛋白过度表达有关。更多还原

【Abstract】 Objective To investigate the protective effect of Rosa Laevigata Mickx on renal lesions in diabetic rats and the mechanism.Methods Sprague-Dawley(SD) rats with diabetes by intraperitoneal injection of STZ were randomly divided into two groups: diabetes model group,and diabetes intervened with Rosa Laevigata Mickx.In addition another two groups were made: normal control group,control group with Rosa Laevigata Mickx.Blood glucose(BG),body weight(BW),kidney weight(KW) and kidney index(KW/BW) were measured.Glomerular structure of kidney were examined by light microscope.The expression of transforming growth factor-β1(TGF-β1) and type Ⅳ collagen in kidney tissue was studied by immunohistochemistry method.Results BG,kidney index(KW/BW) were increased.Kidney pathologic alteration was obvious.And the expression of TGF-β1,type Ⅳ collagen in diabetic rats were significantly increased(P<0.05).24 weeks after the induction of DM,Rosa Laevigata Mickx markedly decreased the blood sugar,and the expression of TGF-β1,type Ⅳ collagen were significantly decreased,and kidney pathologic changes of diabetic rats were improved in the Rosa Laevigata Mickx intervened group,but kidney pathologic alteration was not manifested in control group with Rosa Laevigata Mickx. Conclusion Rosa Laevigata Mickx can protect kidney of streptozotocin-induced diabetic rats.The mechanism may be partly mediated by inhibiting TGF-β1 and type Ⅳ collagen overexpression in kidney.更多还原