【摘要】 目的为研究骨缺损的基因治疗提供实验基础,构建人骨形态发生蛋白2真核表达载体。方法采用双酶切方法克隆载体将骨形态发生蛋白2基因与真核表达载体pcDNA3.1连接,构建pcDNA3.1-BMP2表达基因载体。结果通过测序分析证实,成功构建BMP2真核表达载体。结论成功构建BMP2表达载体。更多还原

【Abstract】 Objective To clone and construct mammal expression vector for human bone morphogenetic protein 2(BMP2),in order to study gene therapy for bone defect disease.Methods Plasmid pSPBMP2 was digested by XbaⅠ and SalⅠ,the BMP2 gene was subcloned into pcDNA3.1.Results The mammal expression plasmid for BMP2,pcDNA3.1-BMP2,had been constructed.The cloned DNA BMP2 gene was confirmed by sequencing.Conclusion The mammal expression plasmid for BMP2 had been successfully constructed.更多还原