【摘要】 目的:观察养血清脑颗粒对小鼠的镇静催眠作用。方法:①50只昆明种雄性小鼠随机分为5组,即生理盐水对照组,阳性对照组及养血清脑颗粒大、中、小剂量组,每组10只,灌胃给药1次后采用抖笼换能器法测定小鼠的自主活动。②另取50只小鼠同法分组,连续灌胃给药3d,2次/d,于末次给药后50min腹腔注射30mg/kg戊巴比妥钠,记录睡眠时间。③另取150只小鼠,分组与给药方法同②,并于末次给药后50min腹腔注射25mg/kg戊巴比妥钠,观察30min后的入睡鼠数。结果:与生理盐水对照组相比,大、中剂量的养血清脑颗粒可减少小鼠的自发活动,大、中、小剂量的养血清脑颗粒与戊巴比妥钠合用可延长小鼠的睡眠时间(P<0.01),大、中、小剂量的养血清脑颗粒与阈下剂量戊巴比妥钠合用,入睡鼠数增加(P<0.05)。结论:养血清脑颗粒有明显的镇静催眠作用,且与戊巴比妥钠有协同作用。更多还原

【Abstract】 Aim:To observe the sedation effects of Yangxueqingnao Granual on mice.Methods:Ⅰ:Fifty mice were allocated into 5 groups:normal saline group,positive control grooup,and Yangxueqingnao Granual high-,middle-,low-dose groups,10 in each group.After being administered by gavage,the spontaneous movement of mice was detected by tremble cage transducer.Ⅱ:Another 50 mice were allocated into 5 groups as above.The mice were administered by gavage for 3 days,twice per day.At 50 min after the last administration,the pentobarbital sodium at 30 mg/kg was given.The sleeping time of mice was recorded.Ⅲ:A total of 150 mice were allocated into 5 groups(30 in each group) and treated as method Ⅱ.At 50 min after the last administration,the pentobarbital sodium at 25 mg/kg was given.The sleeping mice was counted.Results:Yangxueqingnao Granual could reduce the spontaneous movement of mice.The combinatior of Yangxueqingnao Granual and pentobarbital sodium could prolong sleeping time and increase the number of sleeping mice.Conclusion:Yangxueqingnao Granual is effective in sedation especially in combination with pentobarbital sodium.更多还原