【摘要】 目的:探讨头部药物位点注射对缺氧缺血性脑损伤(HIBD)后学习和记忆的影响。方法:选择40d龄Wistar大鼠27只构建HIBD模型,20d后随机分为A、B、C3组(n=9),同时设D组(不构建模型)为正常对照(n=9)。A组采用维生素B1及维生素B12固定于额顶叶处注射,1次/d,共25d;B组采用功能训练(鲍巴斯法);C组不给予处理。50d后4组动物开始水迷宫实验,观察4组动物搜索站台的潜伏期,评价学习记忆情况。结果:A组训练2d后搜索站台的潜伏期短,游泳有规律,路线清晰。B组和C组训练2d后搜索站台的潜伏期长,游泳轨迹杂乱。D组游泳轨迹清晰,潜伏期短。4组大鼠第1~5d水迷宫试验的潜伏期,B、C组均大于A、D组(P均<0.05),A、D组间及B、C组间差异均无统计学意义(P均>0.05)。结论:药物注射疗法可改善脑损伤后的脑功能,提高学习记忆能力。更多还原

【Abstract】 Aim: To study the effects of epicranium medication injection treatment on learning and memory in brain cells of hypoxic-ischemic brain damage(HIBD).Methods: A total of 27 Wistar rats of 40 days-old were selected and used as test animal models of HIBD. Twenty days later, the rats were divided into 3 groups randomly(A,B,C,n=9).Group D was used as normal control group(n=9). Group A was treated with VitB1 and VitB12 by injecting over frontal lobe and apical lobe once a day for 25 days. Group B was treated with functional training (Bobath methods). Group C was not given any treatment.50 days later, water maze experiment was enforced on 4 groups to observe latency of search plat form and evaluate their learning and memory. Results: Group A which were trained for 2 days had clear and regular swim orbit with short latency. Both group B and C had disorder swim orbit with long latency. The latency of group B and C were longer than those of group A and D(P<0.05). And there were no differences between latency of gorup A and D, B and C (P>0.05). Conclusion: Medication injection treatment could improve the function of brain and the capability of learning and memory.更多还原