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光谱法研究钙黄绿素与鲱鱼精DNA的相互作用
Study on the Interaction between Calcein and Herring Sperm DNA by Spectrometry
【摘要】 本实验在接近生理条件下,以吖啶橙(AO)作为光谱探针,采用UV-Vis光谱、荧光光谱、热变性等方法研究了钙黄绿素(CA)与鲱鱼精DNA的作用机制.确定了钙黄绿素(CA)与鲱鱼精DNA之间主要以嵌插方式作用,钙黄绿素(CA)能对鲱鱼精DNA的生物功能产生一定影响.
【Abstract】 The mechianism of interaction between calcein(CA) and herring sperm DNA has been studied by using acridine orange(AO) as a spectral probe with the ways of UV-Vis spectra,fluorescence spectra and melting curve in buffer solution of Tris-HCl(pH=7.00).The experiments comfirmed that calcein with DNA mainly by intercalative and suggest that the biology function of DNA was changed.
【基金】 国家自然科学基金资助项目(No.30572254)
- 【文献出处】 湖南师范大学自然科学学报 ,Journal of Natural Science of Hunan Normal University , 编辑部邮箱 ,2007年01期
- 【分类号】Q78
- 【被引频次】7
- 【下载频次】284