【摘要】 应用PCR-RFLP技术对湖南黔邵花猪280头个体的血浆胆固醇脂转移蛋白(CETP)基因436 bp进行扩增,用限制性内切酶AvaⅡ对其酶切可得到3种基因型.应用最小二乘单因素模型分析不同基因型对初生重、45日龄重、6月龄日重、体高、体长、膘厚的影响.结果显示:CETP基因AvaⅡ酶切位点的各基因型对各生长性状的影响差异显著(P<0.05),对膘厚的影响差异极显著(P<0.01),Aa基因型对初生重、45日龄重、6月龄重、体高、体长等性状的影响较大,AA基因型相对较低.由此可知,CETP基因是影响生长性状的一个重要基因.更多还原

【Abstract】 In the research,436 bp cholesteryl ester transfer protein(CETP) gene partial sequence in Qianshao spotted pigs are successfully amplified by polymerase chain reaction(PCR) after opitimizing the conditions.AvaⅡ was used for association analysis of mutations of CETP.Three genotypes of were detected by AvaII-RFLP in CETP gene fragment.With the least square analysis of model,all genetype of CETP gene had significant influence the traits of birth weight,weight of 45 days and 6 months,height and length of body(P<0.05) and thickness of fat(P<0.01).Aa genetypes of CETP gene had significant higher birth weight,weight of 45 days and 6 months,tallness and length of body than AA genotype in the pigs.Based on the above metioned results,porcine CETP gene had a great effect on the growth traits in Qianshao spotted pigs.更多还原