【摘要】 采用木瓜蛋白酶提取麦麸中的蛋白质,以蛋白提取率为指标,研究了温度、pH值、固液比、酶用量、提取时间对蛋白提取率的影响.用正交实验确定木瓜蛋白酶提取麦麸蛋白较适宜的条件为固液比为1∶10,提取时间为2.5 h,温度为55℃,pH值为7.0,酶与底物比为0.6%,提取率最高达到44.34%.更多还原

【Abstract】 The wheat bran protein is extracted with papain.The effect of temperature,pH value,solid to liquid ratio,enzyme concentration and reactive time on the extraction content is studied.The optimum extracting condition obtained by orthogonal test is:temperature is 55 ℃,pH value is 7.0,solid to liquid ratio is 1/10,enzyme concentration is 0.6% and reactive time is 2.5 hours,and the protein extraction content is up to 44.34%.更多还原