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糖对溶胶-凝胶法固定化脂肪酶CALB的影响

Effect of sugar on lipase CALB entrapped in sol-gel glass matrix

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【作者】 高静; 檀文礼; 黄志红; 李伟杰; 贺莹;

【Author】 GAO Jing,TAN Wenli,HUANG Zhihong,LI Weijie,HE Ying(School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300130,China)

【机构】 河北工业大学化工学院; 河北工业大学化工学院 天津300130; 天津300130;

【Abstract】 Sugar was selected for protecting Candida antarctic lipase B entrapped in sol-gel glass matrix.The activities of immobilized CALB influenced by sucrose,glucose,trehalose,xylose and maltose were investigated.The result showed that trehalose,glucose and xylose were the best protector for lipase CALB.By means of infrared spectrometry and differential scanning calorimeter,the protection mechanism of immobilized CALB by xylose was studied.The results showed that xylose bonded with lipase provided significant improvement in the stability of entrapped CALB.

【关键词】 溶胶-凝胶; 脂肪酶; 固定化; 乳酸乙酯;
【Key words】 sol-gel; lipase; immobilization; ethyl lactate;
【基金】 国家自然科学基金重点项目(20536040);河北省教育厅自然科学研究项目(2002150)~~
  • 【文献出处】 化工学报 ,Journal of Chemical Industry and Engineering(China) , 编辑部邮箱 ,2007年12期
  • 【分类号】TQ28
  • 【被引频次】4
  • 【下载频次】195
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