【摘要】 以枯草芽孢杆菌B 68为出发菌株,经过紫外线、硫酸二乙酯以及紫外线(UV)+硫酸二乙酯(DES)复合诱变方法,从大量突变株中进行筛选,最后共获得8株优良菌株。其中紫外线诱变获3株,编号为U-28、U-43和U-116,它们对香蕉冠腐病的抑菌效果比原始菌株B 68提高了3.55%、4.37%和3.64%;硫酸二乙酯诱变也获得3株,编号为D-24、D-26、D-87,抑菌效果比原始菌株B 68提高了4.22%、8.48%、3.83%;复合诱变最终获得2株突变株,编号为UD-7,UD-93,抑菌效果比原始菌株B 68提高了2.27%、2.86%。经过传代10代后发现突变株比原始菌株B 68更能保持较高的遗传稳定性。更多还原

【Abstract】 In the experiment,Bacillus subtilis B68 was used as a starting strain,using UV,DES and UV+DES mutation methods,a lot of mutants were screened and eight high yield strains were obtained,of which,three were UV radiate mutation strains U-28,U-43 and U-116,three were DES mutation strains D-24,D-26 and D-87,and two were UV+DES compound mutation strains UD-7 and UD-93.As compared with B68 strain,the inhibitory effects of U-28,U-43,U-116,D-24,D-26,D-87,UD-7 and UD-93 increased 3.55%,4.37%,3.64%,4.22%,5.90%,3.83%,2.27% and 2.86%,respectively.After ten generations subculture,the mutation strains could keep higher genetic stability as compared to B68.更多还原