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原位显微红外法检测热胁迫对荒漠植物刺叶墙藓质膜透性的影响
Effects of Thermostress on the Plasma Membrane Permeability of Desert Moss Tortula desertorum Examined by In-Situ Micro-FTIR Analysis
【摘要】 利用显微红外光谱法研究了不同生境下的刺叶墙藓质膜结构及其热胁迫耐性机理。从酯酰基、碳氢化合物红外吸收区域分析表明,刺叶墙藓野生叶片通过加快组织内部碳氢化合物合成速度,减少质膜渗透性,减轻高温对质膜胁迫损伤;室内培养的原丝体和新叶不能及时对热胁迫产生影响。
【Abstract】 A technique based on Fourier transform infrared microscope(FT-IRM) was developed to detect the corresponding changes in chemical composition associated with thermostress among aging tissues of the desert moss Tortula desertorum.The results indicated that wild leaves could accelerate the rate of carbohydrate synthesis and decrease the permeability of plasma membrane during heating at 45 ℃ for 1 h.Those biological responses can alleviate the damage of heat stress,so called thermotolerance.And the ability has a positive relation to the leaf age among various wild leaves.However,the secondary protonema and leaves,cultivated in artificial incubator,were unable to adapt the change of temperature immediately and the permeability of cellular membranes was increased.
【Key words】 Thermotolerance; Micro-FTIR; Protoplasm membrane permeability; Desert moss;
- 【文献出处】 光谱学与光谱分析 ,Spectroscopy and Spectral Analysis , 编辑部邮箱 ,2007年12期
- 【分类号】Q945.7
- 【被引频次】6
- 【下载频次】186