【摘要】 利用葡萄糖溶液的激光拉曼光谱中的特征峰—CCO(1 125 cm-1),以本底溶液水为内标物,组成相对强度,对葡萄糖溶液含量进行了测定研究,其线性范围为0~1.8 mol.L-1,检出限为0.022 7 mol.L-1,共存的KCl和NaCl不引起干扰,用于葡萄糖氯化钠注射液的测定获得了成功。经测定某市售标识量为葡萄糖氯化钠注射液、5%葡萄糖和10%葡萄糖注射液实测平均浓度分别为4.91%,4.93%和9.92%,其回收率分别在71.88%~126.31%,81.02%~124.89%和74.87%~121.32%之间,RSD为5.44%,4.34%和0.94%。该方法具有测定简单、操作方便,无需添加其他任何化学试剂,是一种绿色分析测定方法。更多还原

【Abstract】 In the present paper we report quantitative analysis of glucose using internal standard laser Raman spectra.A good linear correlation was observed between the intensities of the —COO band at 1 125 cm-1 using excition wavelength of 632.81 nm(r=0.998 8) and the glucose concentration over the range 0-1.8 mol·L-1.Band intensities were normalized against an internal standard(water band at 1 643 cm-1),and the limit of detection(L.O.D.) of glucose is 0.022 7 mol·L-1.The interference ions would not influence the quantitative analysis.When this method was used to determine 5% glucose NaCl, 5% glucose,and 10% glucose injections,the result showed that the recoveries are 71.88%-126.31%,81.02%-124.89% and 74.87%-121.32%,and the RSDs are 5.44%,4.34% and 0.94%,respectively.The non destructive,non intrusive nature of the method makes internal standard laser Raman spectra a convenient,accurate,and green quantitative analysis method.更多还原