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荞麦花多糖的提取及含量测定
Determination for the Content of Polysaccharide from Buckwheat Flower
【摘要】 采用热水提取、乙醇沉淀的方法从荞麦花中提取出了水溶性多糖,采用硫酸-苯酚法在波长为497nm处进行了糖含量的测定,求得其校准曲线的回归方程为y=0.00508x+0.0294,相关系数为r=0.9978。平均回收率为99%—103%,RSD为0.74%。结果显示:荞麦花多糖的产率为2.5%,糖含量为41.63%。
【Abstract】 Polysaccharide was extracted from buckwheat flower with hot water-ethanol method and was determined by phenol-sulphuric acid method(λ=497nm).The regression equation is y=0.00508x+0.0294 with correlation coefficient of r=0.9978.The average recovery was 99%—103% with RSD of 0.74%.The recovery of the buckwheat flower polysaccharide is 2.5% and the amount of polysaccharide is 41.63%.
【关键词】 荞麦花多糖;
提取;
分光光度法;
【Key words】 Polysaccharide from Buckwheat Flower; Extraction; Spectrophotometry;
【Key words】 Polysaccharide from Buckwheat Flower; Extraction; Spectrophotometry;
【基金】 内蒙古自然科学基金资助课题,批号为NJ04068
- 【文献出处】 光谱实验室 ,Chinese Journal of Spectroscopy Laboratory , 编辑部邮箱 ,2007年02期
- 【分类号】TS210.7
- 【被引频次】11
- 【下载频次】368