【摘要】 目的:检测增生性玻璃体视网膜病变(PVR)视网膜前膜和视网膜色素上皮(RPE)细胞中组织型转谷氨酰胺酶(tTG)的表达情况,探讨tTG是否参与PVR的发病过程。方法:PVR患者21例,收集视网膜前膜,C级8例,D级13例,行tTG免疫组织化学染色。另对培养3～5代的人RPE细胞分别用含有100mL/LPVR玻璃体液、正常玻璃体液和PBS的DMEM培养液进行孵育24h后行tTG免疫细胞化学染色。光镜下观察,比较C级和D级膜的表达阳性率,显微图像分析测量3组RPE细胞染色的平均灰度值。结果:在PVR视网膜前膜中tTG呈阳性表达(81%),C级和D级膜表达阳性率无差异(C级88%,D级77%,P>0.05)。培养的人RPE细胞表达tTG,在PVR患者的玻璃体液作用下,tTG表达的平均灰度值为137.0±2.6,与正常玻璃体液组(143.5±2.9)及PBS对照组(143.6±3.0)相比,表达水平升高(P<0.05)。结论:PVR视网膜前膜和培养的人RPE细胞tTG表达阳性,在PVR患者的玻璃体液作用下,RPE细胞的tTG表达水平升高,这表明tTG参与了PVR的发病过程,在PVR视网膜前膜的形成过程中可能有重要作用。更多还原

【Abstract】 AIM:To investigate the expression of tissue transglutaminase (tTG) in proliferative vitreoretinopathy (PVR) epiretinal membranes and retinal pigment epithelium (RPE) cells which is thought to be a key element in PVR formation. METHODS:Twenty-one samples of epiretinal membranes were obtained from patients with PVR involved previous rhegmatogenous retinal detachment. Eight samples were at C stage of PVR,13 samples were at D stage. The samples were selected to perform immunohistochemical staining. Thirdto fifth-passage human RPE cells were incubated by serum-free DMEM containing 100mL/L PVR vitreous samples,normal vitreous samples or PBS and stained immunocytochemically. Positive rates of expression were calculated in both C and D stage membranes under light microscopy. The staining average gray values of the three group RPE cells were detected with image analyzing system. RESULTS:The expression of tTG was present in PVR epiretinal membranes (80%). Positive staining was most prominent in ECM,fibroblast-like cells and macrophages. There was no significant difference in positive rates between C and D stage membranes (P >0.05). Cultured human RPE cells expressed tTG,and the expression level of RPE cells incubated with PVR vitreous samples was higher than those incubated with normal vitreous samples and PBS (P <0.05). CONCLUSION:The findings demonstrated that tTG is present in PVR epiretinal membranes and cultured human RPE cells. Its amount is stimulated to increase by the PVR vitreous humor. tTG takes part in the process of PVR. It may play an important role in the formation of PVR membranes.更多还原