【摘要】 目的研究了半胱胺(CS)对油酸(OA)代谢过程中自由基(ROS)生成和ATP酶(ATPase)活性的影响。方法通过培养体外小鼠原代肝细胞,添加不同浓度的OA作用24h,通过luminol化学发光法,TBA法、DTNB法、无机磷比色法分别测定ROS、丙二醛(MDA)、谷胱甘肽(GSH)和Na+-K+-ATP酶活性;选取OA(0,150,250μM/L)三个梯度,添加CS(0,50,100,150,200,250,300,350,400,450,500μM/L),测定相同的指标。结果添加OA大于150μM/L时,ROS、MDA生成量急剧升高,ATPase活性显著降低(P<0.05)。添加CS能提高GSH,减少ROS和MDA的生成,提高ATPase的活性,且作用的效果显著(P<0.05)。但大量添加CS(350-500μM/L)则效果相反。结论大量添加OA会引起肝细胞氧化应激;添加CS能清除ROS,提高ATPase活性;并伴随OA添加量的增加,CS的最佳需要量也增加。更多还原

【Abstract】 Objective To study the effect of cysteamine(CS) on the level of ROS and ATP during the metabolism of oleic acid(OA).Methods Primary hepatic cells were treated with OA(0,50,150,200,250μM/L) for 48h;levels of MDA,GSH and ROS were measured in the hepatic cells by chemiluminescence assay using TBA,DTNB and luminal respectively;the activity of the Na+-K+-ATPase was tested by inorganic phosphorus spectrophotometer.In addition,the hepatic cells treated with OA(0,150,250μM/L) were analysed in the presense of CS(0,50,100,150,200,250,300,350,400,450,500μM/L) for 24h.Results High concentration of OA(150-250μM/L) induced hepatocyte oxidation accompanied by marked increase in ROS and MDA levels and decrease in the activity of ATPase(P<0.05).CS could raise GSH and activity of ATPase, and reduce the production of MDA and ROS.However,high concentrations of CS(350-500μM/L)acted inversely.Conclusion High concentrations of OA can induce lipid oxidation;CS can inhibit it by eliminating ROS and elevating ATPase activity in a dose-dependent manner.更多还原