【摘要】 研究了修饰核苷7-甲基鸟苷(7-Methylguanosine)的电化学行为及测定方法.在pH 1.98的B-R缓冲液中,用循环伏安法(CV),线性扫描伏安法(LSV)、微分脉冲伏安法(DPV)等现代电化学技术研究7-甲基鸟苷在玻碳电极(GCE)上的伏安行为.实验表明,7-甲基鸟苷在+1.036 V(vs.SCE)电位处产生一个阳极氧化峰,峰电流与7-甲基鸟苷的浓度在5.0×10-5-2.0×10-6mol/L范围内呈良好的线性关系,最低检测限(D=2σ/K)为4.1×10-7mol/L.并用恒电位库仑电解法等方法对其氧化机理进行了较为详细的探讨,得到了可能的电极反应机理:7-甲基鸟苷在玻碳电极上的电极反应是属于失1质子和2电子的不可逆的氧化反应.更多还原

【Abstract】 The electrochemical response of 7-methylguanosine in pH 1.98 B-R buffer was investigated by cyclic voltammetry(CV),linear sweep voltammetry(LSV) and differential pulse voltammetry(DPV) method.The differential pules voltammetry exhibited an oxidation current peak of 7-methylguanosine at the potential of +1.036 V(vs.SCE).The peak current is linearly increased with the concentration of 7-methylguanosine in the range of 5.0×10-5~2.0×10-6 mol/L.The detection limit is 4.1×10-7 mol/L.The possible mechanism for the irreversible reaction was assumed to be the oxidation lossing of one proton and two electrons.更多还原