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8种方法显现的汗潜指印STR分型研究
A study on STR typing of human sweat latent fingerprints developed by various methods
【摘要】 目的研究常见指印显现方法对指印STR检验的影响。方法采用Invisorb spin forensic试剂盒提取纯化人汗潜指印DNA,低拷贝模板(LCN)STR复合扩增,荧光电泳检验。结果用铜粉、铝粉、荧光粉、黑磁粉、"502"胶、茚三酮、磺酸双三嗪荧光显色液显现的玻片、纸张和胶带纸粘面上的汗潜指印可成功进行STR分型。结论常见指印显现方法不影响指印STR检验。
【Abstract】 Objective To estimate the effect of various fingerprint phanerosis methods on STR typing of sweat latent fingerprint.Methods The genomic DNA was successfully extracted from sweat latent fingerprint with Invisorb spin forensic kit.STR loci were detected by multiplex PCR amplification and subsequent electrophoresis analysis with ABI 3100 Genetic Analyzer.Results Except developed by silver nitrate,all the sweat latent fingerprint samples developed with copper powder,aluminium powder,fluorescence powder,black magnetic powder,ninhydrin,fluoresent liquid and cyanoatrylate(i.e."502" glue) obtained reliable results of STR genotyping.Conclusion The common fingerprint phanerosis methods do not influence STR genotyping of sweat latent fingerprint except for silver nitrate procedure.
【Key words】 Forensic biological evidence; Sweat latent fingerprint; STR genotyping;
- 【文献出处】 中国法医学杂志 ,Chinese Journal of Forensic Medicine , 编辑部邮箱 ,2007年06期
- 【分类号】D919
- 【被引频次】19
- 【下载频次】453