【摘要】 目的探讨改良扩增前引物延伸(IPEP)法对痕量DNA样本STR检测分型的效果。方法用改良IPEP法对痕量样品DNA进行全基因组扩增(WGA),扩增产物用实时荧光定量PCR技术定量、用AmpFLSTR~ Indentifiler~试剂盒作基因型检测。结果该方法可增加模板DNA约200~1100倍。基因组DNA不低于0.025ng时,可获得15个STR基因座和Amelogenin性别基因座的分型结果。基因组DNA0.01~0.025ng时,可获得9个以上基因座的分型结果。结论改良IPEP法可有效提高痕量DNA样本STR分型检验的灵敏度,有较好的实用价值。更多还原

【Abstract】 Objective To study the effect of modified improved primer extension preamplification (IPEP) on STR analysis of trace DNA.Methods Whole genome amplification (WGA) was performed for trace DNA using modified IPEP, and the amplified products were quantitated by real-time quantitative PCR as well as genotyped by AmpFLSTR~ Indentifiler~ kit.Results The template DNA can be amplified 200 to 1100 folds by modified IPEP. Using this technique, all 15 STR loci and amelogenin gene can be genotyped correctly when the genomic DNA is more than 0.025ng, and more than 9 STR loci can be genotype with success while the genomic DNA is between 0.01ng to 0.025ng.Conclusion Modified IPEP can increase the sensitivity of STR analysis and is helpful for trace DNA analysis.更多还原