【摘要】 以不同剂量的60Coγ射线照射人红白血病细胞系k562细胞,用流式细胞仪检测照射后24小时及不同时间点的细胞周期变化。照后24小时检测结果表明,随着照射剂量的增加,G2/M期细胞比率增加,剂量达15 Gy时,出现明显的G2/M期阻滞;随着剂量的进一步增加,S期细胞比率逐渐增加,剂量达30 Gy时出现明显的S期阻滞;在此剂量(5~30 Gy)下,细胞凋亡未见明显变化。不同时间点的检测结果表明,随着照射剂量的增加,k562细胞从以G2/M期阻滞为主逐渐转化为以S期阻滞为主,同时细胞凋亡也明显增加。更多还原

【Abstract】 The cell cycle change of k562 leukemia cell line was investigated using flow cytometer after k562 cells were irradiated by ⁶⁰Co γ radiation of different dosage.The results from the analysis on cell cycle of k562 cells 24 h after irradiation show that the ratio of G₂/M phase increased as the radiation dosage increased.The arrest of G₂/M phase took place at 15 Gy dose of ⁶⁰Co γ radiation.After that,the radiation dosage increased companied with increasing the rate of S phase.The arrest of S phase took place at 15 Gy dose of ⁶⁰Co γ radiation.The apoptosis did not change in the 5 to 30 Gy range.The results at different time show that the arrest of G₂/M phase was changed to the arrest of S phase along with the increasing dosage,and the apoptosis increased apparently.更多还原