【摘要】 以模式植物拟南芥为材料,采用RT-PCR分析、基因克隆、转化等方法对CIPK1基因功能进行了研究.结果发现,CIPK1(CBL-interacting protein kinase 1)基因在拟南芥茎和花中表达量最高,在叶中表达量最低;ABA能迅速诱导CIPK1基因的表达,GA则抑制该基因的表达,但2,4-D和6-BA对该基因的表达无明显影响;通过对CIPK1基因转基因突变体进行ABA处理,发现转基因拟南芥种子的萌发率明显高于野生型.以上结果表明CIPK1基因的表达具有组织特异性,并且该基因参与ABA和GA信号传导,尤其在ABA促进种子休眠的信号传导途径中具有非常重要的作用.更多还原

【Abstract】 RT-PCR,gene clone,transformation were used to study the function of CIPK1(CBL-interacting protein kinase 1) gene in Arabidopsis.The result showed that CIPK1 gene expressed at high levels in flowers and stems and at low levels in leaves.Moreover,CIPK1 gene can be induced by ABA and inhibited by GA,whereas 2,4-D and 6-BA only had mild effects.In addition,we also found that the germination frequency of CIPK1 was higher than WT respond to ABA.These observations collectively provide initial evidence that CIPK1 gene is likely to be expressed preferentially in some of the tissues and plays an important role in signal pathway of ABA and GA,especially in the pathway of ABA seed dormancy regulation.更多还原