【摘要】 以油菜沪油15幼叶为材料,采用RACE技术克隆获得1条新的抗冷相关基因(BnCOR14,GenBank登录号AY456378).该基因全长为564bp,含有1个387bp的开放阅读框(ORF),编码129个氨基酸的多肽,其理论分子量约为14kD.序列分析表明BnCOR14与拟南芥及荠菜等COR蛋白具有较高的相似性,且BnCOR14具有典型的LEA蛋白序列特征,表明BnCOR14可能在油菜抵抗冷胁迫的过程中具有重要的作用.更多还原

【Abstract】 Using leaves of Brassica napus cv.’Huyou No.15’ as material,RACE-PCR was employed to clone a new cDNA encoding a protein with a calculated molecular weight of about 14 kD from B.napus(BnCOR14,AY456378).The full-length cDNA of BnCOR14 was 564 bp and contained a 387 bp open reading frame(ORF) encoding a 129 amino-acid polypeptides.Sequence analysis showed that BnCOR14 had higher similarity with cold-regulated proteins from Arabidopsis thaliana and Capsella bursa-pastoris and with the typical motifs of late embryogenesis abundant(LEA) proteins,implied that BnCOR14 might have similar functions possessed by other COR proteins in increasing freezing tolerance of plants.更多还原