【摘要】 目的:探讨生长激素(GH)和医用生物蛋白胶(FS)联合应用对腹腔感染肠吻合口愈合的影响机制。方法:将腹腔感染24 h大鼠随机分为三组:对照组行肠切除肠吻合;FS组行肠切除肠吻合,吻合口喷涂FS;GH组行肠切除肠吻合,吻合口喷涂FS,术后应用GH。观察吻合口组织形态学及纤维连接蛋白(FN)、α-平滑肌肌动蛋白(α-SMA)、血管内皮细胞生长因子(VEGF)表达和细胞凋亡情况。结果:GH组FN、α-SMA、VEGF表达均显著高于对照组及FS组,术后吻合口细胞凋亡明显低于对照组和FS组。结论:FS联合应用GH能减轻感染对组织修复起始过程的影响,促进血管内皮细胞增殖及肉芽组织形成,促使成纤维细胞向肌成纤维细胞分化,且α-SMA表达的动态变化过程与肌成纤维细胞凋亡出现的频度呈现出较好的相关性。更多还原

【Abstract】 Objective: To investigate the mechanism of combined growth hormone and fibrin glue on early healing of intestinal anastomosis in intra-abdominal sepsis rats.Methods: Male Wistar rats of abdominal sepsis were randomized into 3 groups.Control rats were given intestinal anastomosis after intestinal fistula operation.Glue group was given intestinal anastomosis and fibrin glue after intestinal fistula.GH group was given intestinal anastomosis and fibrin glue and growth hormone after intestinal fistula.FN,α-SMA,VEGF,and apoptosis in anastomoses were measured.Results: Expression of intestinal FN,α-SMA,and VEGF in GH group were higher than those in other groups and apoptosis were lower than others.Conclusion: It is indicated that combined growth hormone and fibrin glue ameliorates healing of intestinal anastomosis in intra-abdominal sepsis rats.更多还原