【摘要】 目的观察肿瘤坏死因子仅α(NF-α)对大鼠胸主动脉血管平滑肌细胞(VSMC)增殖和syndecan-4蛋白表达的影响。方法体外培养大鼠胸主动脉VSMC,1、10、20、100ng/mL的TNF-α分别作用并设立对照组(不采取干预措施)进行比较,采用MTS/PES法确定VSMC的增殖状态,利用Western blot蛋白免疫印迹法测定syndecan-4蛋白表达。结果各组细胞增殖率分别为对照组1.001±0.064,TNF-α1ng/mL组1.082±0.272,10ng/mL组1.125±0.198,20ng/mL组1.566±0.060.100ng/mL组1.215±0.231。统计分析显示低至1ng/mL的TNF-α仍能明显刺激大鼠VSMC的增殖(与对照组比较,P<0.05),其中以TNF-α20ng/mL组细胞增殖最显著。TNF-α对VSMC的syndecan-4蛋白的表达有显著的增强作用(与对照组比较,P<0.05),其中也以20ng/mL组增强作用最显著,结论TNF-α对体外培养大鼠VSMC增殖和syndecan-4蛋白表达均有显著的促进作用。更多还原

【Abstract】 Objective To investigate the effect of tumor necrosis factor-α(TNF-α)on proliferation of vascular smooth muscle cell(VSMC)and expression of syndecan-4 protein in rat thoracic aorta.Methods Rat VSMC were exposed to TNF-αwith different concentrations(1,10,20 and 100 ng/mL,respectively)and cultured for 24h in vitro,in addition to the control group established for comparison.The ratio of VSMC proliferation was determined by non-radioactive MTS/PES assay and the expression of syndecan-4 protein was evaluated by Western blotting using anti-syndecan-4 antibody. Results The ratios of cell proliferation were 1.001±0.064 in the control group,and 1.082±0.272, 1.125±0.198,1.566±0.060 and 1.215±0.231 respectively in TNF-α-treated groups with the corresponding TNF-αconcentration of 1,10,20 and 100 ng/mL.Statistical analysis showed that TNF-αsignificantly stimulated the proliferation of rat VSMC with the concentration even as low as 1ng/mL(compared with control group,P<0.05),especially significantly with the concentration of 20 ng/mL.The expression of syndecan-4 protein in VSMC was significantly enhanced by TNF-α(compared with control group,P<0.05),also especially significantly with the concentration of 20 ng/mL.Conclusion TNF-αcan stimulate proliferation of in vitro cultured rat VSMC and expression of syndean-4 protein.更多还原