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中草药对牙鲆病原迟钝爱德华氏菌的体外抑制作用研究
In Vitro Antibacterial Activity of Chinese Herbs to Pathogenic Edwardsiella tarda Isolated from Flounder(Paralichthys olivaceus)
【摘要】 用倍比稀释法测定了30种中草药对牙鲆病原迟钝爱德华氏菌(LN031012-8,TH030908-5)菌株的体外抗菌活性。试验结果表明,五倍子、诃子、黄芩、秦皮和红藤的抑菌作用明显,最小抑菌浓度(MIC)<12.5 mg/ml,最小杀菌浓度(MBC)<50 mg/ml;丁香、穿心莲、虎杖、夏枯草等有一定的抑菌作用,MIC为50~100 mg/ml;而茵陈、鱼腥草、金银花、大青叶等的抑菌作用不明显,MIC均>100 mg/ml;五倍子、地榆、野菊花、马齿苋等中草药的体外抗菌活性表现了株间差异。
【Abstract】 The antibacterial activity of 30 Chinese herbs to pathogenic Edwardsiella tarda(LN031012-8,TH030908-5)isolated from Japanese flounder(Paralichthys olivaceus)were determined in vitro by microdilution procedure.The results showed that Galla Chinensis,Fructus chebulae,Radix scutellariae,Cortex fraxini and Caulis sargentodoxae were found to have significant inhibition of the bacterium with minimal inhibitory concentration(MIC)less than 12.5 mg/ml and minimal bactericidal concentration(MBC)less than 50 mg/ml;Flos caryophylli,Herba andrographitis,Rhizoma polygoni cuspidate,and Spica prunellaee showed some extent inhibition of the bacterium with MIC of 50~100 mg/ml,but Herba artemisiae scopariae,Herba houttuyniae,Flos lonicerae,and Folium isatidis had no inhibition of the bacterium with MIC of more than 100 mg/ml.There was difference in antibacterial activity between the strains(LN031012-8,TH030908-5)to Galla Chinensis,Radix sanguisorbae,Flos chrysanthemi indici,and Herba portulacae.
【Key words】 Chinese herb; Edwardsiella tarda; minimal inhibitory concentration(MIC); minimal bactericidal concentration(MBC);
- 【文献出处】 水产科学 ,Fisheries Science , 编辑部邮箱 ,2007年05期
- 【分类号】S943
- 【被引频次】27
- 【下载频次】370