【摘要】 目的观察运动疲劳对大鼠海马和纹状体脑源性神经营养因子(BDNF)及胶质纤维酸性蛋白(GFAP)表达的影响,探讨运动疲劳产生的神经生物学机制。方法:选取24只SD大鼠随机分为对照组(CG)和实验组(EG),分别在运动后0,12,24 h将大鼠麻醉,灌注制备脑石蜡切片,采用免疫组化染色法观察并分析海马和纹状体BDNF、GFAP表达水平的变化。结果EG海马锥体细胞排列紊乱疏松,部分细胞出现固缩、肿胀和碎裂;GFAP阳性细胞胞体增大,突起分支增多、增粗,着色加深。海马和纹状体12EG、24EG组BDNF阳性表达显著高于CG(P<0.05,P<0.01),且海马24EG显著高于0EG(P<0.05)。各EG大鼠海马区神经细胞GFAP阳性表达较CG明显增强(P<0.05,P<0.01)。结论运动疲劳引起大鼠海马和纹状体BDNF和GFAP表达水平上调,提示BDNF与GFAP参与了运动疲劳产生的神经生物学调控过程。更多还原

【Abstract】 To investigate BDNF and GFAP expression changes in rats’ hippocampus and striatum after swimming and discuss the neurobiology mechanism of exercise-induced fatigue,24 rats were divided randomly into CG and EG(0EG,12EG,24EG) and BDNF and GFAP expression changes in hippocampus and striatum were observed using SABC method.After exercise-induced fatigue,cells arrange shows foul-up and loosen in cone district of hippocampus.Some cells crinkle,strut and chip.GFAP expression in cell’s body becomes augment and shows wider axon.Expression of BDNF in 12EG,24EG significantly increased compared with CG(P <0.05,P <0.01),and 24EG also significantly increased compared with 0EG(P <0.05).Expression of GFAP significantly increased compared with CG(P <0.05,P <0.01).Exercise-induced fatigues have increased BDNF and GFAP expression.It has been proved that both of BDNF and GFAP may play an important role in the neurobiological modulation for exercise-induced fatigue.更多还原