【摘要】 为了解红腹锦鸡、石鸡和雉鸡的血象、血液生理生化参数正常值及其特点,为人工饲养繁殖与健康检测提供参考资料,采用改良纽巴氏法、沙利氏比色法、离心法、瑞氏染色等方法和全自动血液生物化学分析仪对红腹锦鸡、石鸡和雉鸡的血象和16项生化指标进行了测定,通过SDS-PAGE分析了血浆蛋白质。结果表明:红腹锦鸡的平均红细胞体积、淋巴细胞、嗜酸性粒细胞、嗜中性粒细胞、血浆总蛋白、血浆白蛋白、血浆钠和血浆氯在性别之间有显著差异,所测其它生理生化指标在性别之间无显著差异;红腹锦鸡的红细胞、平均红细胞体积、凝血细胞、尿素氮和肌肝与石鸡和雉鸡存在显著差异,红腹锦鸡与石鸡的白细胞存在显著差异,所测其它生理生化指标在三者之间无显著差异。种属不同可能是导致3种雉科鸟类血液生理常值差别的主要原因,而采样时的机体状况、营养状况及测试操作方法也有一定的影响。更多还原

【Abstract】 To explore hemogram,the normal values and characteristic of biochemical and physiological in blood of Chrysolophus pictus,Alectoris chukar and Phasianus colchicus,and to provide the reference data for artificial feeding,propagation and health examination,sixteen physiological and biochemical indices of blood in Chrysolophus pictus,Alectoris chukar and Phasianus colchicus were determined by adopting refind Neubauer method,Sahlis method,centrifugal method,Wright staining method as well as automatic biochemical analyser.Plasma protein was also analyzed by SDS-PAGE.Results showed that significant differences were observed in the mean corpuscular volume,the number of lymphocyte,acidophilic granulocyte and neutrophil granulocyte,the concentrations of total protein and albumin,inorganic natrium and inorganic chlorine in plasma in Chrysolophus pictus of different sexes,but no significant differences were found in the other indices of different sexes.A comparison of Chrysolophus pictus with Alectoris chukar and Phasianus colchicus showed that significant differences existed in the number of red blood cell,mean corpuscular volume,thrombocyte,urea nitrogen and creatinine in plasma,significant difference was observed in the number of white blood cell between Chrysolophus pictus and Alectoris chukar,but no significant differences were found in the other indices.The differences in the biochemical and physiological values can be attributed to such factors as their different species,different organism,different nutrition conditions and the determination methods.更多还原