【摘要】 目的应用计算机信号采集和处理技术研究豚鼠离体心肌细胞正常、缺血及再灌注时的电生理特性。方法离体细胞灌流,细胞内微电极记录,计算机信号采集与处理。结果豚鼠右心室乳头肌肌细胞静息电位RP为-72±6.58 mV,动作电位峰值(APA)为112.22±8.65 mV,0相最大去极化速率(Vmax)为107.37±10.55 mV.s-1。复极达峰值电位的10%、50%、90%所需时间(APD10、APD50、APD90)分别为39.25±8.55 ms、123.45±15.25 m s和169.35±18.12 ms。缺血缺氧台氏液灌流的心肌细胞APA与Vmax降低,APD90显著缩短,与缺血缺氧前相比有显著性意义(P<0.05或P<0.01)。再灌注2 min内可见AP缺血缺氧样变化加重,Vmax继续降低,与缺血缺氧灌流相比有显著性意义(P<0.01)。豚鼠再灌注10 min后RP与AP波形基本恢复正常。结论计算机信号采集和处理技术在心肌细胞电生理研究方面的应用,为全面的分析心肌细胞生物电的变化及其形成机制,亦为深入研究病理及药物作用情况下对生物电的影响提供真实客观的数据。更多还原

【Abstract】 Aim To investigate the electrophysiological character of normal,ischemic and reperfusion of ventricular myocardium in vitro in guinea pig by means of computer signal acquisition and processing technique.Methods Superfusion of ventricular myocardium in vitro and intracellular recording technique were adopted.Results The mean value of resting potential(RP) of ventricular myocardium of guinea pig was(-72±6.58)mV.The mean value of action potential amplitude(APA) was 112.22±8.65 mV.The maximum of depolarization velocity(Vmax) was 107.37±10.55 mV·s-1.The action potential duration at 10%,50% and 90%(APD10,APD50 and APD90) was 39.25±8.55 ms,123.45±15.25 ms and 169.35±18.12 ms respectively.After superfusion by ischemia and hypoxia Tyroad solution,the APA and Vmax decreased and APD90 shortened significantly.After reperfusion 2 minutes,The Vmax continuing to decrease significantly showed that the effects of ischemic aggravated.After reperfusion 10 minutes there was RP and AP reablement on the whole.Conclusion As for research of electrophysiological character of ventricular myocardium,the computer signal acquisition and processing technique could be used as a powerful tool to obtain objective and real data for analyzing the mechanism of changing and formation of cell bioelectricity or investigating more about effects of pathology and drug on electrophysiological properties of myocardium.更多还原