【摘要】 选用无角道塞特、萨福克与中国美利奴肉羊品种群体,耳组织提取基因组DNA,用4对微卫星引物进行PCR扩增,通过电泳分型、凝胶成像系统分析各位点等位基因及全部个体的标记基因型,计算基因频率、多态信息含量(PIC)和杂合度等,从分子水平上分析4个位点的遗传多态性。结果表明,CSSM18,OB2,MCM38,MCMA26这4个位点的等位基因数分别为14、151、3、13,多态信息含量(PIC)丰富,杂合度高,均属于高度多态性位点,用于作为与生产性能相关的遗传标记是较理想的。更多还原

【Abstract】 In this study,a group of 161 sheep consisting of Dorset,Suffolk and Chinese Merino for meat was chosen and its population genetic polymorphism was analyzed at the level of molecule with the process of genome DNA extraction from ear issue,PCR amplification of 4 primers,fragment typing,allele of every locus and genotype analysis of all individuals with Gel Imaging system,calculation of allele frequencies polymorphism information content(PIC)and Heterozygosity.The results showed that,the number of alleles in CSSM18,OB2,MCM38 and MCMA26 was14,15,13 and 13;the polymorphism information content(PIC)was abundant,and the heterozygosity was high.All of them were high polymorphicloci.So they were idealloci genetic markers related to the production performance.更多还原