【摘要】 目的探讨参附注射液对大鼠移植胰缺血/再灌注损伤的保护作用,并分析其中的机制。方法正常SD大鼠6只为对照组,糖尿病SD大鼠24只随机分为4组,每组6只。缺血/再灌注组(I/R组)、参附预处理组(SF组)、红参预处理组(HS组)和附子预处理组(FZ组)。除对照组外各组均行胰腺移植,24只SD大鼠为供体。SF、HS和FZ组在移植前1日及术前30 min经静脉分别予受体注射参附注射液(10 mg/ kg)、红参注射液(9 mg/kg)、附子注射液(1 mg/kg),对照组和I/R组在移植前1日及术前30 min经静脉予受体注射同等容积生理盐水。检测各组再灌注前、后血糖；再灌注后2 h血清中TNF-α和NO的含量、移植胰组织中SOD,MPO和MDA含量；用TUNEL法观察移植胰组织细胞凋亡情况,Western Blot法检测移植胰组织Bax和Bcl-2蛋白表达情况。结果再灌注后SF组、HS组和FZ组较I/R组血糖低、血清中TNF-α含量低、NO含量高；再灌注后SF组、HS组和FZ组较I/R组移植胰组织中SOD活性高、MDA含量低、MPO活性低、凋亡指数低、Bcl-2表达高、Bax表达低、Bcl-2/Bax比值高。结论参附注射液时大鼠移植胰的缺血/再灌注损伤具有保护作用,机制可能是提高SOD的活性、增加内源性NO的合成、减少TNF-α分泌、减轻PMNs黏附与聚集、上调Bcl-2和下调Bax基因表达。更多还原

【Abstract】 Objective To investigate the protective effect of Shenfu Injection against ischemia-reperfusion (L/R)injury due to pancreas transplantation in rats,and explore its possible mechanism.Methods Six normal SD rats with sham operation were taken as the normal control group,24 steptozozin-induced diabetic SD rats were randomly divided into 4 groups,with 6 in each group.Except I/R group,the rats in the other groups were intrave- nous injected with Shenfu Injection0(SF,10 mg/kg),Hongshen Injection(HS,9mg/kg)and Fuzi Injection(FZ 1 mg/kg)respectively at the day and 30 minutes before pancreas transplantation performed in the SF group,HS group and FZ group,respectively.At the same time,rats in the normal control group and in the I/R group were intravenously injected the same volume of normal saline.The blood glucose was detected before and after reperfu- sion,and 2 hours later after reperfusion,the contents of serum nitric oxide(NO)and tumor necrosis factor-alpha (TNF-α),the concentrations of malondialdehyde(MDA),superoxide dismutase(SOD),and myeloperoxidase (MPO)in the transplanted pancreas tissues were detected.The cell apoptosis of the transplanted pancreas tissue was determined by TUNEL,and the bcl-2 and Bax protein expression was determined by Western blot.Results After reperfusion,the levels of blood glucose and TNF-αdecreased and the concentration of NO increased in the SF group,HS group and FZ group,compared with those in the I/R group.The activity of SOD,bcl-2 expression and the ratio of bcl-2 and Bax were higher,while the content of MDA,the activity of MPO,apoptotic indexes,and Bax expression were lower in the SF group,HS group and FZ group than those in the I/R group.Conclusion Shenfu Injection can protect I/R injury due to pancreas transplantation in rats,the possible mechanism may be related to promoting activity of SOD,increasing synthesis of endogenous NO,decreasing the excretion of TNF-α,alleviating conglutination and aggregation of polymorphonuclear neutrophils(PMNs)in pancreas,as well as up-regulating Bcl-2 gene expression and down-regulating the Bax gene expression.更多还原