【摘要】 目的研究电针“合谷”穴(LI4)对大鼠局灶脑缺血/再灌注后室下区(SVZ)神经干细胞增殖的影响。方法采用“线栓法”建立大鼠局灶脑缺血/再灌注模型,将88只Wistar大鼠随机分为正常对照组(4只)、假手术组(4只)、模型组和电针组,其中模型组和电针组按1、7、14、21、28天5个时间点分为5个亚组,每个亚组8只。取大鼠双侧“合谷”穴作为刺激部位,用电针治疗仪作连续刺激,每次刺激时间为15min,每天1次,7天为1个疗程。免疫组化法检测SVZ区神经上皮干细胞蛋白(Nestin)的表达。结果与正常对照组比较,模型组中再灌注1天时梗塞侧SVZ Nestin阳性细胞开始增加,7天达到高峰(P＜0．05),14天后开始下降,28天后接近正常；模型组7天梗塞灶周围出现大量Nestin阳性细胞。与模型组比较。电针组(SVZ)Nestin阳性细胞在再灌注后1天时开始增加,7天时达到高峰,与模型7天组比较,统计学分析显示增加约1．5倍(P＜0．05),14天后开始下降,28天后接近正常；电针刺激7天后梗塞灶周围Nestin阳性表达较模型7天组增强。结论脑缺血可激活内源性神经干细胞的自我增殖潜能,而电针刺激后可使内源性神经干细胞的增殖更加明显。更多还原

【Abstract】 Objective To investigate the effect of electroacupuncturing （EA） Hegu point （LI4） on prolifer- ation of neural stem cells in the subventricular zone （SVZ） after rat focal ischemia/reperfusion （I/R）.Methods Animal model was made by ligating external carotid artery and inserting a piece of nylon thread into the internal ca- rotid artery.Eighty-eight Wistar rats were randomly divided into the normal control group （n=4）,the sham opera- ted group （n=4）,the I/R groups （contain 1,7,14,21,28 days’five time points,each point n=8）,and the I/R +EA group （contain 1,7,14,21,28 days’five time points,each point n=8 ）.Bilateral Hegu points on rat were consecutively stimulated for 15 min,once daily,and 7 days as one treatment course.The protein expression of Nestin in SVZ was detected by immunochemistry method.Results Compared with the normal control group,the number of Nestin positive cell in SVZ in the I/R group increased strikingly on the 1^st day,peaked on the 7^th day after reperfu- sion （P＜0.05）,began to decrease on the 14^th day,and almost reached normal on the 28^th day.A number of Nestin positive ceils were present around the ischemic core in the cortex on the 7^th day after reperfusion.After performed electroacupuncture,the number of Nestin positive cells in SVZ in the EA group increased on the 1^st day,peaked on the 7^th day,and increased 1.5 times as compared with the I/R 7 days group,the difference was obvious （P＜0.05）,and began to decrease on the 14^th day,and almost reached normal on the 28^th day.Nestin immunoreactivi- ty around the ischemic core in the cortex was obviously presented in the EA 7 days group.Conclusion The results indicate that cerebral ischemia can stimulate the potent of self-proliferation of neural stem cells and electroacupunc- ture can obviously increase the proliferation of neural stem cell.更多还原