【摘要】 目的观察抗脑血栓片对缺血性脑损伤的保护作用,并初步探讨其可能的作用机制。方法大鼠灌胃给予抗脑血栓片,连续14天,每天2次。采用大鼠大脑中动脉栓塞(MCAO),制作急性局灶性脑梗塞动物模型,观察缺血24h后对脑梗塞体积和神经行为学变化的影响,以及对大鼠体外血栓形成、血小板聚集、血液黏度等指标的影响。结果抗脑血栓片可依剂量性减轻大鼠局灶性脑缺血所致的脑梗塞体积以及改善由脑缺血引起的神经症状。大、中、小剂量组脑梗塞体积分别为(11．18±3．35)%、(14．60±7．00)%和(15．37±7．21)%,与模型组(27．51±4．71)%比较,各组依次分别下降59．36%、46．93%和44．13%(P＜0．01),各剂量组对体外血栓的干、湿重量均亦可明显减轻,与模型组比较,差异有显著性(P＜0．05,P＜0．01),可显著抑制ADP诱导的血小板聚集,降低全血黏度,但对血浆黏度、红细胞压积无明显影响。结论抗脑血栓片对脑缺血有明显的保护作用,其作用可能与它改善血液黏度、抑制血小板聚集有关,对其确切作用机制,有待深入研究。更多还原

【Abstract】 Objective To observe the protective effect of Kang Naoxueshuan Tablet (KNT) on ischemic brain injury in rats,and explore its possible mechanism.Methods Rats were administrated with KNT twice per day for successive 14 days.Rat model of acute focal cerebral ischemia was established by middle cerebral artery occlusion (MCAO) with a nylon suture inserted through the right internal carotid artery to occlude the beginning of middle cerebral artery.After 24 hrs MCAO,the neurological deficit and the volume of cerebral infarct were ob- served,and the effect of KNT on the thrombosis of rats in vitro,platelet aggregation and blood viscosity were also determined.Results KNT could alleviate volume of cerebral infarct caused by focal cerebral ischemia in a dose- dependent manner and improve neurological symptoms.The volume of cerebral infarct was 11.18±3.35%,14.60 +7.00% and 15.37±7.21% in the high,middle and low-dose groups,respectively,and they were decreased 59.36%,46.93% and 44.13% than that in the model group 27.51±4.71% (P＜0.01 ).The wet and dry weigh of thrombosis in vitro of the three different dose groups were significantly decreased,and they were significantly dif- ferent than that of the model group (P＜0.05,P＜0.01).KNT could significantly inhibit platelet aggregation in- duced by ADP and decrease blood viscosity,but it had no effect on plasma viscosity and hematocrit.Conclusion KNT has significant protective effect on ischemia,the mechanism is relateed to the improvement of blood viscosity and inhibiti on of platelet aggregation.But the exact mechanisms need to be probed into deeply.更多还原