【摘要】 目的:探讨抑癌基因PTEN和凋亡抑制基因survivin在宫颈腺癌中的表达情况及其与人乳头瘤病毒(HPV)感染的关系。方法:采用组织微阵列技术结合原位杂交和免疫组化二步法检测HPV16/18DNA和PTENs、urvivin蛋白在86例宫颈腺癌和24例慢性宫颈炎组织中的表达。结果:HPV16/18DNA与survivin蛋白在宫颈腺癌中的阳性表达率分别为65.1%与67.4%,均显著高于慢性宫颈炎组织(P<0.01)。HPV16/18感染与宫颈腺癌的病理分级和组织学类型无关,但与survivin表达呈明显正相关(P<0.05)。survivin表达与宫颈腺癌的病理分级有关,G2、G3组阳性表达率均明显高于G1组(P<0.01)。PTEN与survivin表达呈明显负相关(P<0.01)。透明细胞腺癌和子宫内膜样腺癌survivin阳性表达率均明显高于宫颈黏液型腺癌(P<0.05)。结论:宫颈腺癌的发生与HPV16/18感染及PTEN、survivin蛋白的表达密切相关;三者在宫颈腺癌的发病机制中可能起着协同作用。更多还原

【Abstract】 Objective:To study the expressions of PTEN and survivin in cervical adenocarcinoma,and to analyze the relationship between the infection of human papilloma virus(HPV) and the expressions of PTEN and survivin.Methods: The expressions of HPV16/18 DNA and PTEN and survivin proteins were determined by tissue microarray combined with in situ hybridization and immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammation.(Results:)The positive rates of HPV16/18DNA and survivin protein in cervical adenocarcinoma were 65.1% and 67.4%,respectively,significantly higher than that in cervical chronic inflammation(P<0.01).HPV16/18 infection had no correlation with the pathological grades or histological classfication,but had positive correlation with survivin expression(P<0.05).The expression of survivin was related to the pathological grades of cervical adenocarcinoma.The positive rate of survivin was significantly higher in G2 and G3 group than in G1 group(P<0.01).PTEN expression negatively correlated with survivin expression(P<0.05).The expression level of survivin was significantly higher in clear-cell adenocarcinoma and endometrioid adenocarcinoma than that in mucinous adenocarcinoma(P<0.05).Conclusion:The occurrence of cervical adenocarcinoma may be related to the infection of HPV16/18 and the abnormal expressions of PTEN and survivin.They might play a synergistic action in the nosogenesis of cervical adenocarcinoma.更多还原