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RP-HPLC法测定垂阴茶糖浆中岩白菜素的含量
Determination the Content of Bergenin in Chuiyincha Syrups by RP-HPLC
【摘要】 目的:测定垂阴茶糖浆中岩白菜素的含量。方法:采用反相高效液相色谱法,色谱柱:VP-ODS(150 mm×4.6 mm,5μm),流动相:甲醇-水(15:85);检测波长:275 nm;流速:1.0 ml·min-1;柱温:室温。结果:岩白菜素的线性范围0.25~2.52μg,r=0.999 9,平均回收率为98.76%,RSD%=1.3(n=6)。结论:该法适用于测定垂阴茶糖策中岩白菜素的含量。
【Abstract】 Objective:To set up a RP-HPLC method for the determination of content of Bergenin in Chuiyincha syrups. Method: The VP-ODS column(150 mm×4.6 mm,5μm) was used. The mobile phase consisted of methanol-water (15:85), and the detection wavelength was at 275nm. Result:The linear range of Bergenin was 0.25 - 2.52μg(r = 0.999 9). The average recovery was 98.8%,RSD = 1.3% (n = 6). Conclusion:The method can be used for the determination the content of Bergenin in Chuiyincha syrup.
【关键词】 垂阴茶糖浆;
岩白菜素;
反相高效液相色谱法;
含量测定;
【Key words】 Chuiyincha syrup; Bergenin; RP-HPLC; Content determination;
【Key words】 Chuiyincha syrup; Bergenin; RP-HPLC; Content determination;
- 【文献出处】 中国药师 ,China Pharmacist , 编辑部邮箱 ,2006年11期
- 【分类号】R286.0
- 【下载频次】33