【摘要】 目的:研究高脂饮食肝损伤中结构型(cNOS)、诱导型(iNOS)一氧化氮(NO)合成酶及凋亡相关基因Bax、Bcl-2表达的变化,在进一步探讨脂肪性肝损伤机制的同时观察黄连素的干预调控作用。方法:建立高脂饮食大鼠肝损伤模型,ig给予黄连素20,40,60 mg·kg-1,测定血中TC,TG,HDL,LDL含量及ALT,GST活性和肝中NO含量;免疫组化方法观察cNOS,iN- OS,Bax,Bcl-2的表达。结果:黄连素可使血中TC,TG,LDL含量及sALT,sGST活性下降,呈良好的剂量依赖关系,对HDL无明显作用;能显著抑制肝组织中NO的生成;黄连素对高脂饮食大鼠肝组织中iNOS,Bax表达有一定的抑制作用,但作用无显著性差异;可显著上调肝组织中cNOS及Bcl-2的表达,但不呈剂量依赖关系。结论:黄连素可保护南脂饮食所致的肝损伤,可显著上调抗凋亡基因Bcl-2的表达,提示黄连素可能通过抗肝细胞凋亡保护高脂饮食引起的肝细胞损伤。更多还原

【Abstract】 Objective: To investigate the modulation of Berberine on the expression of nitric oxide synthase and Bax, Bcl-2 in liver injury mice treated by high fat-high cholesterol diet. Method: Liver injury model was induced by high-fat-high-cholesterol. Berberine was administrated to mice with 20, 40, 60 mg·kg-1 , respectively, ig, once a day. Mice were sacrificed at the end of the 6th week, the effects of Berberine on the expression of inducible, constitutive nitric oxide synthase and Bax, Bcl-2 gene were assessed by immuno his to chemistry , and the nitric oxide (NO) production, alanine transaminase(sALT) activity,glutathione S-transferase(sGST) activity in serum were determined. Result: Compared with the normal mice, the NO production,TC、TG、LDL content and sALT, sGST level were increased significantly, no change of HDL was found; iNOS, Bax expression were up-regulation, cNOS expression and Bcl-2 expression were down-regulation in liver injury mice. The Berberine could obviously reduce NO production and sALT, sGST level, increase the expression of cNOS and Bcl-2, and no effect on iNOS, Bax expression was found. Conclusion: Berberine can alleviate the liver injury by modulation of the expression of cNOS, Bcl-2 to prevent apoptosis.更多还原