【摘要】 白术外植体诱导及植株再生试验结果表明:NAA为白术叶片、叶柄愈伤组织诱导和芽分化的主导因子;MS+BA 1.0 mg/L+NAA 0.3 mg/L+GA30.2 mg/L为白术叶片、叶柄愈伤组织诱导、芽分化的最佳培养基;腋芽增殖的培养基以MS+BA 0.3 mg/L+IBA 0.5 mg/L较为理想,诱导率达95%;1/2MS+IBA 0.1~0.5 mg/L为白术试管苗生根的最佳培养基,生根率在90%以上;试管苗移栽成活率达90%以上。更多还原

【Abstract】 A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz..The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation.MS+BA 1.0 mg/L+NAA 0.3 mg/L+GA₃ 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also.MS+BA 3.0 mg/L+IBA 0.5 mg/L was suitable for the proliferation of axillary buds,with an induction rate of 95%.1/2MS+IBA 0.1⁰.5 mg/L was optimum for rooting,with a rooting percentage more than 90%.The survival rate of transplanted plantlets was more than 90%.更多还原