【摘要】 目的:探讨经穴输氧治疗大鼠颅脑损伤的作用机制。方法:SD大鼠50只被随机分为正常组、假损伤对照组、电针组(取百会、足三里、阳陵泉、三阴交穴电针治疗)、常规治疗组(给予仙台合剂4.17 m l/kg)、经穴输氧组(将氧气按0.1 L/m in输入穴位)5组。后3组大鼠制备脑损伤模型。各组除给予常规治疗外进行对应治疗,7 d后活杀动物取双侧脑组织,检测脑组织一氧化氮合酶(NO S)活性变化,并进行脑组织病理观察。结果:与假损伤对照组比较,各治疗组可显著降低伤侧及对侧脑组织NO S活性,差异有显著性(P<0.05或P<0.01);且经穴输氧组伤侧NO S活性显著低于电针组和常规治疗组(P<0.05和P<0.01)。治疗前创伤各组双侧大脑半球不对称,伤侧半球明显肿胀,挫伤中心区有出血、神经细胞坏死,挫伤周边神经组织水肿,细胞肿胀,毛细血管塌陷,有血管外出血;治疗后各组基本为正常脑表现。结论:经穴输氧对治疗脑损伤后遗症有效,可以改善脑部血供,纠正脑组织缺血、缺氧状态,具有保护神经细胞作用。更多还原

【Abstract】 Objective: To study the mechanism of the oxygen therapy through acupunctures on rats with brain injury.Methods: Fifty SD rats were randomly divided into five groups: normal,sham injury,(electro-acupuncture)(at Baihui(百会),Zusanli(足三里),Yanglingquan(阳陵泉),Sanyinjiao(三阴交)),routine treatment(Xiantai mixture(仙台合剂) 4.17 ml/kg) and oxygen therapy through acupunctures((0.1 liter) per minute groups.Brain injury model was established in the latter three groups. Besides the routine treatment in all the groups,the corresponding therapies were given to the treatment groups(respectively).The rats were killed 7 days after the respective treatment,the two hemispheres of the brain(were)(taken) out.Changes of nitric oxide synthase(NOS) activity and pathologic changes of brain tissues were(observed).Results: Comparing with the sham injury group,all treatment groups had a more obvious decrease of NOS activity,the differences being significant(P<0.05 or P<0.01),and the last group which was offered oxygen therapy through acupunctures had even obviously lower level of NOS activity than those of the other two treatment groups: the electro-acupuncture and routine treatment groups (P<0.05 and P<0.01).Before treatment,the two hemispheres in the groups with injuries were not symmetric,pathologic changes showed that the injured side was obviously swollen,at the center of the lesion,there were blood and necrosis of nerve cells,and at the periphery of the lesion,the nerve tissues were edematous,nerve cells swollen,capillary(vessels) dilated with extra-vascular bleeding.After treatment,normal brain manifestations were seen in each group.Conclusion: Oxygen therapy through acupunctures is effective for the treatment of the sequelae of brain injuries,it may improve the blood and oxygen supply to correct the ischemia and hypoxia in the brain tissues and protect the nerve cells.更多还原