【摘要】 目的:研究肝细胞癌(HCC)患者乙型肝炎病毒(HBV)基因型与基本核心启动子(BCP)基因区A1762T/G1764A双位点变异(BCP区双突变)之间的关系,探讨HCC的发病机制。方法:选择40例HCC患者作为研究组,40例慢性乙型肝炎(CHB)患者作为对照组,采用多对型特异性引物PCR扩增法进行基因分型及HBV基因多态性芯片检测BCP区A1762T/G1764A双位点变异。结果:40例HCC患者中有30例HBV DNA定量阳性,平均对数值为(6·53±1·31)copy/ml,将30例HBV DNA定量阳性的HCC及40例CHB患者的血清进行HBV基因分型及基因变异检测,结果显示30例HCC中HBV B基因型5例(16·7%),C基因型25例(83·3%);BCP区双突变共有20例(67·7%),其中B基因型1例,C基因型19例,BCP双突变率在B基因型和C基因型中分别为20%(1/5)和76%(19/25);40例CHB患者中B基因型32例(80%),C基因型8例(20%);BCP区双突变共有13例,其中B基因型8例,C基因型5例,BCP双突率在B基因型和C基因型中分别是25%(8/32)和62·5%(5/8)。结论:肝细胞癌的发生与BCP区A1762T/G1764A双位点变异有关,多发生在HBV C基因型的患者。更多还原

【Abstract】 Objective:To investigate the relationships between hepatitis B virus (HBV) genotype and HBV core promoter mutants, and in order to explore the pathogenesis of hepatocellular carcinoma.Methods:Serum samples from 40 patients with hepatoma and 40 patients with chronic hepatitis B(CHB) were collected and tested for HBV genotypes by the multiplex type-specific primers, Signal-base mutations of G1896A. A1762T and G1764A were analyzed by gene chips technique.Results:There were 30 cases in the 40 HCC patients whose HBV DNA quantification were positive, the average levels of the HBV DNA quantification were (6.53±1.31) copy/ml(Logarithm).The HBV genotypes and HBV mutants were tested for the 30 HCC and 40 CHB patients, the results showed that in the 30 HCC cases, 5(16.7%) were genotype B, 25 (83.3%) were genotype C; Twenty of the 30 HCC patients were found to have mutants in HBV BCP region at nucleotide A1762T/G1764A,among which 1 case were detected with genotype B and 19 cases with genotype C (20% VS 76%,P<0.05).In the 40 CHB patients, the percentagens of genotype B and C were 80%,20% respectively,13 of 40 CHB patients were found to have mutants in BCP region, among which 8 cases were genotype B and 5 cases with genotype C.Conclusion:The genotype C is the predominant genotype in HBV related HCC, and there are a clear link between HBV genotype and HBV BCP mutants at nucleotide A1762T/G1764A in hepatoma.更多还原