【摘要】 目的动态观察黄连素对兔颈动脉球囊损伤后血清一氧化氮(NO)、血浆内皮素-1(ET-1)水平的影响,并探讨其改善血管内皮功能的可能机制。方法日本大耳白兔40只,随机分为假手术组、模型组、黄连素组和辛伐他汀组,除假手术组外各组均以球囊导管扩张损伤颈动脉内膜,并分别给予生理盐水、黄连素注射液和辛伐他汀配制液2.5mg/(kg.d)腹腔注射,假手术组仅普食喂养。分别于术后3、7及15d探测各组右侧颈总动脉直径,并于耳缘静脉采血,检测血清NO含量及血浆ET-1浓度。结果术后3、7及15d,黄连素组血清NO含量与假手术组无明显差异,但显著高于模型组及辛伐他汀组(P<0.01);黄连素组和辛伐他汀组血浆ET-1水平与假手术组均无明显差异,但显著低于模型组(P<0.01);术后15d,黄连素组和辛伐他汀组血管直径与假手术组均无明显差异,但显著大于模型组(P<0.01)。结论黄连素可通过抑制兔颈动脉球囊损伤后NO浓度降低及ET-1水平升高,改善动脉损伤后血管内皮功能。更多还原

【Abstract】 Objective To observe the effect of berberine on the levels of nitric oxide(NO) and endothelium-1(ET-1) after balloon withdrawal injury of rabbit carotid artery and evaluate the mechanism of improving endothelial function. Methods 40 Japanese rabbits were allocated randomly into 4 groups: artificial group(n=7),model group(n=11),berberine group(n=11) and simvastatin group(n=11). We made balloon endothelium denud-ation in carotid artery of rabbits in the later three groups in anesthesia. They were injected respectively with 0.9% sodium chloride,berberine and simvastatin liquid(2.5mg/kg.d). The diameter of carotid artery,NO and ET-1 of peripheral blood of all groups were detected after 3 days,7 days and 15 days. Results On 3rd day,7th day and 15th day,elevation of NO of berberine group was unconspicuous compared with that in artificial group but was significant compared with that in model group and simvastatin group(P<0.01); the ET-1 levels of berberine group and simvastatin group were markedly lower than those in model group (P<0.01),but have no obvious difference with artificial group. On 15th day,the vascular diameters of berberine group and simvastatin group have no significant difference with artificial group,but obviously increased compared with model group(P<0.01). Conclusion Berberine can improve vascular endothelial function after percutaneous transluminal angioplasty by inhibiting reduction of NO concentration and elevation of ET-1 levels.更多还原