【摘要】 采用共培养法研究了不同温度下青枯雷尔氏菌强致病力菌株F-01-V与无致病力菌株F-01-A之间的生长竞争、生防菌ANTI-8098A胞外物质对F-01-V和F-01-A生长的影响以及对F-01-V与F-01-A之间生长竞争关系的影响。结果表明,在15、20、25、30和35℃,菌株F-01-V、F-01-A单独静置培养48h后,F-01-V活菌数的增长率最高可达123.7%,F-01-A的活菌数下降,最大降幅为60.7%;当F-01-V与F-01-A等量混合培养时,F-01-V能继续增长,但48h后的增长率不如单独培养时的高,F-01-A的生长受到促进,使得这两个菌株的活菌数之比(F-01-V/F-01-A)与混合前的比值(0.97)相近;生防菌胞外物质对F-01-V有较强的抑制作用,抑制率与温度呈正相关,对F-01-A具有促进生长的作用,促长率也与温度呈正相关;生防菌胞外物质对F-01-V的抑制作用和对F-01-A的促进生长作用,导致在生防菌液、F-01-V与F-01-A共存的环境中,F-01-V与F-01-A之间的生长竞争向着有利于F-01-A的方向发展,使F-01-A成为优势菌株,特别是在30℃以上的温度下,F-01-V/F-01-A小于0.01,F-01-A的优势极为明显。更多还原

【Abstract】 The present paper dealt with the growth competition between the virulent strain F-01-V and avirulent strain F-01-A of Ralstonia solanacearum, and the effects of the extracellular substances of bacterial-wilt-disease biocontrol strain ANTI-8098A, Bacillus cereus on the growths of strains F-01-V and F-01-A as well as influences of strain ANTI-8098A on the growth competitions at different temperatures. The results showed that the numbers of strain F-01-V increased with the largest increasing rate of 123.7%, that of strain F-01-A decreased with the largest decreasing rate of 60.7%, when they were separately maintained at temperatures 15, 20, 25, 30 and 35℃ for 48h, respectively. While they were in mixing cultures of strain F-01-V and F-01-A for 48h, the growth of strain F-01-V was inhibited and the growth of strain F-01-A was facilitated. The extracellular substances of strain ANTI-8098A could inhibit the growth of strain F-01-V, but it could facilitate the growth of strain F-01-A. There was a positive correlation be-tween the inhibiting (or the facilitating) rate and temperature. When the mixed cultures were maintai- ned at temperatures at 30℃, the ratio values of F-01-V/F-01-A were less than 0.01, which indicated the strain F-01-A were significantly dominated.更多还原