【摘要】 目的观察外源性非酶糖基化终产物(AGEs)对大鼠视网膜单核细胞趋化蛋白-1(MCP-1)表达的影响。方法应用鼠血清白蛋白(RSA)体外孵育AGEs修饰蛋白,并将其注入健康大鼠体内,每日一次,连续两周。取大鼠视网膜行HE染色及MCP-1免疫组织化学检查。结果AGEs组大鼠视网膜内核层,神经节细胞层细胞排列疏松,细胞间隙增大,细胞数减少。MCP-1在大鼠视网膜内核层,神经节细胞层部分细胞的胞浆中呈阳性表达,AGEs组表达明显增强,其平均灰度值为126.83±2.91,同正常对照组及RSA组相比,差别有统计学意义。结论AGEs可作为独立因素导致视网膜组织损害,并引起正常大鼠视网膜组织中MCP-1表达增高,进而共同参与糖尿病视网膜病变的发展。更多还原

【Abstract】 Objective To investigate the influence of advanced glycated end products(AGEs) on the expression of monocyte chemotactic protein-1(MCP-1).Methods AGEs were prepared by incubating rat serum albumin(RSA) with D-glucose,then infused it to healthy rats.Immunohistochemical method was performed to detect the expression of MCP-1 with positive staining identified by a brown precipitate.Results The constitution of retina is clear in the rats among the AGE-RSA group.The cells of inner nuclear layer and ganglion cell layer loosened,intercellular space largened,cells amount decreased.The brown precipitate,located in the inner nuclear layer and ganglion cell layer,indicates the presence of MCP-1.Positive cells were significant increased in AGE-RSA group.The average gray value of retina specimens staining for MCP-1 was 126.83±2.91 which was significanthigher than that of the two control groups(P<0.01).Conclusion Advanced glycation end products can induced the higher expression of MCP-1 in the retina of healthy rats.Both have some relationship with the development of the diabetic retinopathy.更多还原