【摘要】 目的将编码艾滋病病毒(HI V-1)外膜蛋白的env基因、核蛋白的gag基因与编码干扰素(IFNα-2b)基因分别插入pSFJ16与pSFJ38真核表达载体,观察表达产物诱导机体产生细胞免疫的变化规律。方法利用分子生物学技术构建重组质粒,经质脂体转染与野生型痘苗病毒在Cos-7细胞内同源重组,筛选、纯化获得重组痘苗病毒vJ38gag/IFNα-2b和vJ16env/IFNα-2b。免疫小鼠后,检测小鼠外周血与脾淋巴细胞对ConA及Lps的反应性,用流式细胞仪测定小鼠脾细胞CD4+、CD8+,细胞计数。结果外周血与脾淋巴细胞对ConA及Lps的反应性,实验组与对照组比较有显著差异(P<0.05)。CD4+T淋巴细胞与对照组比较有显著差异(P<0.05)。CD8+T淋巴细胞与对照组比较无显著差异(P>0.05),但呈增高趋势。结论重组痘苗病毒能诱导小鼠产生较强的细胞免疫。重组痘苗病毒能在体外与HI V-1env和HI V-1gag阳性血清发生特异性反应,具有免疫原性和免疫反应性。更多还原

【Abstract】 Objective To observe the i mmune reaction that induced by the Expression of HI V-1 env,gag and IFNα-2bFusion Genes in Eukaryotic Cell in mice body cell.Methods By liposome transfection,four recombinant virus strain,vJ16env/IFNα-2b,vJ38gag/IFNα-2b,vJ16env and vJ38gag were obtained by HA-plaque screening.The mices were i mmu-nized with the four strain recombinant vaccinia virus,1×107/mice.OD value of HI V-1env and HI V-1gag antibody was de-termined.CD4+.CD8+Tcell figure of mice spleen was examined by Flow Cytometry(FCM).Results There were signif-cant differences(P<0.05),btween OD value.CD4 +T cell counts and CD4+/CD8+ratio of the group and the contrivegroup.Study demostrated :The its i mmungenicity and i mmunereposibility was proved and may be significant in further re-searchin this area.Conclusion Recombinant vaccinia virus caninduce powerly cellular i mmune reaction by vaccinating intomice body and have reaction to serum with HI V-1 env、gagin test,Its i mmungenicity andi mmunereposibility was proved.更多还原