【摘要】 本试验以鱼肠道上皮细胞为模型,以肠道的致病鳗弧菌为对照,研究了丁酸梭菌对鱼肠道上皮细胞的黏附作用及黏附对细胞的损伤和成活率的影响。结果表明∶丁酸梭菌对鱼的肠道上皮的黏附率为7.39±1.85,低于鳗弧菌的黏附率(P<0.05);对细胞的损伤率为1.02±0.35,显著低于鳗弧菌对细胞的损伤率(P<0.05);黏附后丁酸梭菌组对细胞的成活率的影响与正常对照组比较,差异不显著;当酪酸菌、鳗弧菌与肠道上皮细胞共同培养时,使鳗弧菌的黏附率下降7.03%(P<0.05)。因此,该株丁酸梭菌可以安全地黏附鱼肠道上皮细胞,并能有效抑制鳗弧菌的黏附。更多还原

【Abstract】 The intestinal epithelial cell of fish was used as the model to evaluate the adherence rate of Clostridium butyricum and the affluences of adherence to the cell damage and viability.The Vibrio anguillarum treatment was used as the control.Results indicated that the adherence rate of C.butyricum to intestinal epithelial cells was(7.39±1.85) which was lower than that of the control(P<0.05);the cell damage rate of C.butyricum to intestinal epithetical cells was 1.02±0.35 and significantly lower than that of the control(P<0.05);there was not significant different in the cell viability rate of C.butyricum treatment in comparison with the treatment without the bacteria after the C.butyricum was adhered to the epithelia;the cell adherence rate of V.anguillarum decreased 7.03%(P<0.05) when the V.anguillarum and C.butyricum co-culture(P<0.05).In brief,C.butyricum could adhered to the intestinal epithelial cell and inhibit the adherence of V.anguillarum.更多还原