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小鼠骨髓间充质干细胞(MSCs)的分离纯化及其定向诱导分化为神经元样细胞

Isolation and Purificiation of Mouse Bone Marrow Mesenchymal Stem Cells(MSCs) and Their Differentiation into Neuron-Like Cells

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【作者】 胡亮董雅娟柏学进马保华杨恕玲淡新刚董方圆王雪红李建栋程明

【Author】 HU Liang 1,2,DONG Ya-Juan 2*,BAI Xue-Jin2,MA Bao-hua1,YANG Shu-ling2,DAN Xin-gang 1,2,DONG Fang-Yuan2,WANG Xue-hong2,LI Jian-dong2,CHENG Ming2 (1. Key Laboratory of Animal Reproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China, Northwest University of Agriculture and Forestry, Yangling, Shaanxi 712100, China;2. The Center of Animal Embryo Engineering, Lai yang Agriculture College, Laiyang, Shandong 265200,China)

【机构】 西北农林科技大学家畜生殖内分泌与胚胎工程农业部重点开放实验室莱阳农学院胚胎工程中心莱阳农学院胚胎工程中心 陕西杨凌712100莱阳农学院胚胎工程中心山东莱阳265200陕西杨凌712100

【摘要】 比较了全骨髓法和密度梯度离心法分离、纯化小鼠骨髓间充质干细胞,并研究了不同培养基和血清对干细胞生长的影响以及小鼠骨髓间充质干细胞的神经分化潜能。结果表明,密度梯度离心法和贴壁法结合使用可以分离纯化小鼠骨髓间充质干细胞;所获得的小鼠骨髓间充质干细胞具有诱导分化为神经元样细胞和脂肪样细胞的潜能,为小鼠骨髓间充质干细胞体外定向诱导分化的研究提供了技术支撑。

【Abstract】 The present experiments compared whole-marrow isolation with density gradient isolation for the purification of MSCs, and investigated the influence of different culture media and sera on culture of MSCs, together with their ability of differentiation into neuron-like cells. It was proved that density gradient isolation combined with adherence isolation can purify MSCs, which was able to differentiate into neuron-like cells and adipogenic cells as well. Accordingly it provides a powerful technical support for the futher study of MSCs differentiate into neuron like cells.

【基金】 山东省自然科学基金(Y2005D01);教育部重点科研项目(01608)
  • 【文献出处】 中国兽医学报 ,Chinese Journal of Veterinary Science , 编辑部邮箱 ,2006年06期
  • 【分类号】Q813
  • 【被引频次】2
  • 【下载频次】252
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